MSCV PIG C/EBPa
(Plasmid #66985)

• Purpose: Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP
• Depositing Lab: Christopher Vakoc
• Publication: Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 10.1016/j.molcel.2015.04.011.

Backbone

• Vector backbone: MSCV PIG
• Backbone manufacturer: Addgene Plasmid #18751
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 7380
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C/EBP alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 1080
• Entrez Gene: Cebpa (a.k.a. C/ebpalpha, CBF-A, Cebp)
• Promoter: LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: MSCV

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry