pYFP-ErbB2
(Plasmid #66948)

• Purpose: Mammalian expression of rat ErbB2 tagged with YFP
• Depositing Labs: Mads Lerdrup, Bo van_Deurs
• Publication: Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. Epub 2007 Jul 11.

Backbone

• Vector backbone: pcDNA3.1D/V5-His-TOPO
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ErbB2
• Alt name: HER2
• Species: R. norvegicus (rat)
• Mutation: (See depositor comment below on mutations A24T and V1259A)
• Entrez Gene: Erbb2 (a.k.a. HER2/neu, neu)
• Promoter: CMV
• Tags / Fusion Proteins:
  • YFP (N terminal on insert)
  • V5 Tag (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A24T and V1259A are genetic variations and have no effect on function.