perbB1Y1045F-EGFP
(Plasmid
#40267)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 40267 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-N3
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameerbB1
-
Alt nameEGFR
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3630
-
MutationTyr1045>Phe
-
Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
perbB1Y1045F-EGFP was a gift from Martin Offterdinger (Addgene plasmid # 40267 ; http://n2t.net/addgene:40267 ; RRID:Addgene_40267) -
For your References section:
Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane. Offterdinger M, Bastiaens PI. Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13. 10.1111/j.1600-0854.2007.00665.x PubMed 17956594