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Addgene

perbB1Y1045F-EGFP
(Plasmid #40267)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40267 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    erbB1
  • Alt name
    EGFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3630
  • Mutation
    Tyr1045>Phe
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    perbB1Y1045F-EGFP was a gift from Martin Offterdinger (Addgene plasmid # 40267 ; http://n2t.net/addgene:40267 ; RRID:Addgene_40267)
  • For your References section:

    Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane. Offterdinger M, Bastiaens PI. Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13. 10.1111/j.1600-0854.2007.00665.x PubMed 17956594