alpha actinin delta ABD-GFP
(Plasmid #66935)

• Purpose: Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted
• Depositing Labs: Pere Roca-Cusachs, Michael Sheetz
• Publication: Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1361-70. doi: 10.1073/pnas.1220723110. Epub 2013 Mar 20.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Alpha-actinin 1
• Species: H. sapiens (human)
• Mutation: actin binding domain (amino acids 30–253) is deleted
• Entrez Gene: ACTN1 (a.k.a. BDPLT15)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry