Skip to main content
Addgene

pTER RB1shRNA human
(Plasmid #66886)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66886 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pTER
  • Backbone manufacturer
    A gift from Dr. Marc van de Wetering (PMID: 12776180)
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    retinoblastoma 1
  • gRNA/shRNA sequence
    GAACGATTATCCATTCAAA
  • Species
    H. sapiens (human)
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Promoter H1 Tet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pQE promoter
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTER RB1shRNA human was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 66886 ; http://n2t.net/addgene:66886 ; RRID:Addgene_66886)

  • For your References section:

    Tumors with nonfunctional retinoblastoma protein are killed by reduced gamma-tubulin levels. Ehlen A, Rossello CA, von Stedingk K, Hoog G, Nilsson E, Pettersson HM, Jirstrom K, Alvarado-Kristensson M. J Biol Chem. 2012 May 18;287(21):17241-7. doi: 10.1074/jbc.M112.357038. Epub 2012 Apr 6. 10.1074/jbc.M112.357038 PubMed 22493456
Related items:
Commonly requested with: