pCDNA3 humanN-Flag SadBS
(Plasmid
#66881)
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PurposeMammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 6478
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSadB
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SpeciesH. sapiens (human)
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Entrez GeneBRSK1 (a.k.a. hSAD1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 humanN-Flag SadBS was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 66881 ; http://n2t.net/addgene:66881 ; RRID:Addgene_66881) -
For your References section:
The nuclear localization of gamma-tubulin is regulated by SadB-mediated phosphorylation. Eklund G, Lang S, Glindre J, Ehlen A, Alvarado-Kristensson M. J Biol Chem. 2014 Aug 1;289(31):21360-73. doi: 10.1074/jbc.M114.562389. Epub 2014 Jun 18. 10.1074/jbc.M114.562389 PubMed 24942739