pAV5S-F30-2xdBroccoli
(Plasmid #66845)

• Purpose: Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells
• Depositing Lab: Samie Jaffrey
• Publication: Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 10.1002/9780470559277.ch150174.

Backbone

• Vector backbone: pAV-5S
• Backbone size w/o insert (bp): 6064
• Total vector size (bp): 6298
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Two dimeric Broccoli aptamers in the F30 scaffold
• Species: Synthetic
• Insert Size (bp): 234
• Promoter: 5S
• Tag / Fusion Protein:
  • 5S rRNA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Seq-pAV-F

Resource Information

• Supplemental Documents:
  • Supplemental Information
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry