pAVU6+27-F30-2xdBroccoli
(Plasmid #66842)

• Purpose: Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells
• Depositing Lab: Samie Jaffrey
• Publication: Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi: 10.1016/j.chembiol.2015.04.018.

Backbone

• Vector backbone: pAVU6+27
• Backbone size w/o insert (bp): 6151
• Total vector size (bp): 6385
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Two dimeric Broccoli aptamers in the F30 scaffold
• Species: Synthetic
• Insert Size (bp): 234
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Seq-pAV-F

Resource Information

• Supplemental Documents:
  • Supplemental Information
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry