130_pSKB3-LUMP-P.leioghnati
(Plasmid #65894)

• Purpose: Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime
• Depositing Lab: Gerard Marriott
• Publication: Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.

Backbone

• Vector backbone: pSKB3
• Backbone size w/o insert (bp): 5245
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LUMP
• Species: Photobacterium leignathi
• Insert Size (bp): 633

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • 130_pSKB3-LUMP-P.leioghnati genbank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry