pDONR221_UBE2V1_3UTR
(Plasmid
#65864)
-
PurposeCarries 3' UTR of UBE2V1, an RBPMS target
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepDONR221
-
Backbone manufacturerLife Technologies, modified by the Tuschl lab
- Backbone size w/o insert (bp) 2550
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameUBE2V1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1644
-
Entrez GeneUBE2V1 (a.k.a. CIR1, CROC-1, CROC1, UBE2V, UEV-1, UEV1, UEV1A)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Primers used to amplify 3' UTR from human cDNA (HCC1964 cDNA):
UBE2V1 forward: AAA AAG CAG GCT TCT CAA AAA GAA AAA CCA CAG
UBE2V1 reverse: AGA AAG CTG GGT GTT AAA TAT TAA CGC ACA CT
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDONR221_UBE2V1_3UTR was a gift from Thomas Tuschl (Addgene plasmid # 65864 ; http://n2t.net/addgene:65864 ; RRID:Addgene_65864)