pcDNA3.1/Hygro(-)-B2-TS5
(Plasmid
#104354)
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PurposeExpresses human beta 2 integrin tension sensor
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1/Hygro(-)
- Backbone size w/o insert (bp) 5596
- Total vector size (bp) 9403
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman integrin b2 with tension sensor
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Alt nameITGB2
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SpeciesH. sapiens (human)
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Insert Size (bp)3891
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Entrez GeneITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NHeI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer BGH_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: this plasmid is identical to b2-TS5 (Plasmid #103878).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/Hygro(-)-B2-TS5 was a gift from Pontus Nordenfelt & Timothy Springer (Addgene plasmid # 104354 ; http://n2t.net/addgene:104354 ; RRID:Addgene_104354) -
For your References section:
Coordinated integrin activation by actin-dependent force during T-cell migration. Nordenfelt P, Elliott HL, Springer TA. Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119. 10.1038/ncomms13119 PubMed 27721490