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BPK1520 Citations (38)

Originally described in: Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK Nature. 2015 Jun 22. doi: 10.1038/nature14592. PubMed Journal

Articles Citing BPK1520

Articles
CRISPR-Cas9 Mediated Labelling Allows for Single Molecule Imaging and Resolution. Khan AO, Simms VA, Pike JA, Thomas SG, Morgan NV. Sci Rep. 2017 Aug 16;7(1):8450. doi: 10.1038/s41598-017-08493-x. PubMed
Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature. 2017 Sep 20. doi: 10.1038/nature24268. PubMed

Associated Plasmids
Enhancer SINEs Link Pol III to Pol II Transcription in Neurons. Policarpi C, Crepaldi L, Brookes E, Nitarska J, French SM, Coatti A, Riccio A. Cell Rep. 2017 Dec 5;21(10):2879-2894. doi: 10.1016/j.celrep.2017.11.019. PubMed
Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Grunewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. PubMed

Associated Plasmids
Allele-Specific CRISPR-Cas9 Genome Editing of the Single-Base P23H Mutation for Rhodopsin-Associated Dominant Retinitis Pigmentosa. Li P, Kleinstiver BP, Leon MY, Prew MS, Navarro-Gomez D, Greenwald SH, Pierce EA, Joung JK, Liu Q. CRISPR J. 2018 Feb;1(1):55-64. doi: 10.1089/crispr.2017.0009. PubMed
A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9. Maji B, Gangopadhyay SA, Lee M, Shi M, Wu P, Heler R, Mok B, Lim D, Siriwardena SU, Paul B, Dancik V, Vetere A, Mesleh MF, Marraffini LA, Liu DR, Clemons PA, Wagner BK, Choudhary A. Cell. 2019 May 2;177(4):1067-1079.e19. doi: 10.1016/j.cell.2019.04.009. PubMed
CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung JK. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. PubMed

Associated Plasmids
Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed

Associated Plasmids
Modeling Niemann-Pick disease type C in a human haploid cell line allows for patient variant characterization and clinical interpretation. Erwood S, Brewer RA, Bily TMI, Maino E, Zhou L, Cohn RD, Ivakine EA. Genome Res. 2019 Dec;29(12):2010-2019. doi: 10.1101/gr.250720.119. Epub 2019 Nov 21. PubMed

Associated Plasmids
Insight into Nephrocan Function in Mouse Endoderm Patterning. Addeo M, Buonaiuto S, Guerriero I, Amendola E, Visconte F, Marino A, De Angelis MT, Russo F, Roberto L, Marotta P, Russo NA, Iervolino A, Amodio F, De Felice M, Lucci V, Falco G. Int J Mol Sci. 2019 Dec 18;21(1):8. doi: 10.3390/ijms21010008. PubMed
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Grunewald J, Zhou R, Lareau CA, Garcia SP, Iyer S, Miller BR, Langner LM, Hsu JY, Aryee MJ, Joung JK. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. PubMed

Associated Plasmids
Diaphanous-related formin mDia2 regulates beta2 integrins to control hematopoietic stem and progenitor cell engraftment. Mei Y, Han X, Liu Y, Yang J, Sumagin R, Ji P. Nat Commun. 2020 Jun 23;11(1):3172. doi: 10.1038/s41467-020-16911-4. PubMed
Prime editing for functional repair in patient-derived disease models. Schene IF, Joore IP, Oka R, Mokry M, van Vugt AHM, van Boxtel R, van der Doef HPJ, van der Laan LJW, Verstegen MMA, van Hasselt PM, Nieuwenhuis EES, Fuchs SA. Nat Commun. 2020 Oct 23;11(1):5352. doi: 10.1038/s41467-020-19136-7. PubMed
Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q. Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, Kalfon J, Yuan C, Dumont N, Gonzalez A, Abdusamad M, Li YY, Spurr LF, Wu WW, Durbin AD, Wolpin BM, Piccioni F, Root DE, Boehm JS, Cherniack AD, Tsherniak A, Hong AL, Hahn WC, Stegmaier K, Golub TR, Vazquez F, Aguirre AJ. Cell Rep. 2020 Dec 15;33(11):108493. doi: 10.1016/j.celrep.2020.108493. PubMed

Associated Plasmids
PrimeDesign software for rapid and simplified design of prime editing guide RNAs. Hsu JY, Grunewald J, Szalay R, Shih J, Anzalone AV, Lam KC, Shen MW, Petri K, Liu DR, Joung JK, Pinello L. Nat Commun. 2021 Feb 15;12(1):1034. doi: 10.1038/s41467-021-21337-7. PubMed
Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression. Cheng L, Li Y, Qi Q, Xu P, Feng R, Palmer L, Chen J, Wu R, Yee T, Zhang J, Yao Y, Sharma A, Hardison RC, Weiss MJ, Cheng Y. Nat Genet. 2021 Jun;53(6):869-880. doi: 10.1038/s41588-021-00861-8. Epub 2021 May 6. PubMed
Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation. Edmondson C, Zhou Q, Liu X. BMC Biotechnol. 2021 Jul 27;21(1):45. doi: 10.1186/s12896-021-00707-5. PubMed
Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids. Geurts MH, de Poel E, Pleguezuelos-Manzano C, Oka R, Carrillo L, Andersson-Rolf A, Boretto M, Brunsveld JE, van Boxtel R, Beekman JM, Clevers H. Life Sci Alliance. 2021 Aug 9;4(10). pii: 4/10/e202000940. doi: 10.26508/lsa.202000940. Print 2021 Oct. PubMed
Modelling of primary ciliary dyskinesia using patient-derived airway organoids. van der Vaart J, Bottinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, Eitan K, Gileles-Hillel A, Clevers H. EMBO Rep. 2021 Dec 6;22(12):e52058. doi: 10.15252/embr.202052058. Epub 2021 Oct 25. PubMed
Mutation-specific reporter for optimization and enrichment of prime editing. Schene IF, Joore IP, Baijens JHL, Stevelink R, Kok G, Shehata S, Ilcken EF, Nieuwenhuis ECM, Bolhuis DP, van Rees RCM, Spelier SA, van der Doef HPJ, Beekman JM, Houwen RHJ, Nieuwenhuis EES, Fuchs SA. Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3. PubMed

Associated Plasmids
The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes. Pizzolato G, Moparthi L, Soderholm S, Cantu C, Koch S. J Cell Sci. 2022 Oct 1;135(19):jcs260082. doi: 10.1242/jcs.260082. Epub 2022 Oct 10. PubMed
A novel intergenic enhancer that regulates Bdnf expression in developing cortical neurons. Brookes E, Martinez De La Cruz B, Boulasiki P, Au HYA, Varsally W, Barrington C, Hadjur S, Riccio A. iScience. 2022 Dec 1;26(1):105695. doi: 10.1016/j.isci.2022.105695. eCollection 2023 Jan 20. PubMed
In vitro genome editing activity of Cas9 in somatic cells after random and transposon-based genomic Cas9 integration. Sollner JH, Sake HJ, Frenzel A, Lechler R, Herrmann D, Fuchs W, Petersen B. PLoS One. 2022 Dec 30;17(12):e0279123. doi: 10.1371/journal.pone.0279123. eCollection 2022. PubMed
Delete_In situ architecture of Opa1-dependent mitochondrial cristae remodeling. Fry MY, Navarro PP, Qin X, Inde Z, Ananda VY, Lugo CM, Hakim P, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH. bioRxiv. 2023 Feb 15:2023.01.16.524176. doi: 10.1101/2023.01.16.524176. Preprint. PubMed
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy. Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA, Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ, Tsai SQ, Kothary R, Kleinstiver BP. Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z. PubMed

Associated Plasmids
Functional Assessment of a New PBX1 Variant in a 46,XY Fetus with Severe Syndromic Difference of Sexual Development through CRISPR-Cas9 Gene Editing. Mary L, Leclerc D, Labalme A, Bellaud P, Mazaud-Guittot S, Dreano S, Evrard B, Bigand A, Cauchoix A, Loget P, Lokchine A, Cluzeau L, Gilot D, Belaud-Rotureau MA, Jaillard S. Genes (Basel). 2023 Jan 20;14(2):273. doi: 10.3390/genes14020273. PubMed
Effect of rapamycin on lysosomal accumulation in a CRISPR/Cas9-based cellular model of VPS13A deficiency. Tornero-Ecija AR, Navas MA, Munoz-Braceras S, Vincent O, Escalante R. J Cell Mol Med. 2023 Jun;27(11):1557-1564. doi: 10.1111/jcmm.17768. Epub 2023 May 10. PubMed
Generation of three CRISPR/Cas9 edited human induced pluripotent stem cell lines (DHMi005-A-5, DHMi005-A-6 and DHMi005-A-7) carrying a Holt-Oram Syndrome patient-specific TBX5 mutation with known cardiac phenotype and a FLAG-tag after exon 9 of the TBX5 gene. Lahm H, Stieglbauer S, Neb I, Doppler S, Schneider S, Dzilic E, Lange R, Krane M, Dressen M. Stem Cell Res. 2023 Jun;69:103123. doi: 10.1016/j.scr.2023.103123. Epub 2023 May 16. PubMed
Generation of sheep with defined FecB(B) and TBXT mutations and porcine blastocysts with KCNJ5(G151R/+) mutation using prime editing. Zhou S, Lenk LJ, Gao Y, Wang Y, Zhao X, Pan M, Huang S, Sun K, Kalds P, Luo Q, Lillico S, Sonstegard T, Scholl UI, Ma B, Petersen B, Chen Y, Wang X. BMC Genomics. 2023 Jun 12;24(1):313. doi: 10.1186/s12864-023-09409-y. PubMed
PMS1 as a target for splice modulation to prevent somatic CAG repeat expansion in Huntington's disease. McLean ZL, Gao D, Correia K, Roy JCL, Shibata S, Farnum IN, Valdepenas-Mellor Z, Rapuru M, Morini E, Ruliera J, Gillis T, Lucente D, Kleinstiver BP, Lee JM, MacDonald ME, Wheeler VC, Pinto RM, Gusella JF. bioRxiv [Preprint]. 2023 Jul 27:2023.07.25.550489. doi: 10.1101/2023.07.25.550489. PubMed
One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids. Geurts MH, Gandhi S, Boretto MG, Akkerman N, Derks LLM, van Son G, Celotti M, Harshuk-Shabso S, Peci F, Begthel H, Hendriks D, Schurmann P, Andersson-Rolf A, Chuva de Sousa Lopes SM, van Es JH, van Boxtel R, Clevers H. Nat Commun. 2023 Aug 17;14(1):4998. doi: 10.1038/s41467-023-40701-3. PubMed
A biallelic multiple nucleotide length polymorphism explains functional causality at 5p15.33 prostate cancer risk locus. Spisak S, Tisza V, Nuzzo PV, Seo JH, Pataki B, Ribli D, Sztupinszki Z, Bell C, Rohanizadegan M, Stillman DR, Alaiwi SA, Bartels AH, Papp M, Shetty A, Abbasi F, Lin X, Lawrenson K, Gayther SA, Pomerantz M, Baca S, Solymosi N, Csabai I, Szallasi Z, Gusev A, Freedman ML. Nat Commun. 2023 Aug 23;14(1):5118. doi: 10.1038/s41467-023-40616-z. PubMed
Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases. Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, Kleinstiver BP. Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x. PubMed

Associated Plasmids
Engineered transcription-associated Cas9 targeting in eukaryotic cells. Goldberg GW, Kogenaru M, Keegan S, Haase MAB, Kagermazova L, Arias MA, Onyebeke K, Adams S, Fenyo D, Noyes MB, Boeke JD. bioRxiv [Preprint]. 2023 Sep 18:2023.09.18.558319. doi: 10.1101/2023.09.18.558319. PubMed
The FXR1 network acts as signaling scaffold for actomyosin remodeling. Chen X, Fansler MM, Janjoš U, Ule J, Mayr C. Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015. PubMed

Associated Plasmids
Benzomorphan and non-benzomorphan agonists differentially alter sigma-1 receptor quaternary structure, as does types of cellular stress. Couly S, Yasui Y, Foncham S, Grammatikakis I, Lal A, Shi L, Su TP. Cell Mol Life Sci. 2024 Jan 9;81(1):14. doi: 10.1007/s00018-023-05023-z. PubMed
iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease. Busquets O, Li H, Mohieddin Syed K, Jerez PA, Dunnack J, Bu RL, Verma Y, Pangilinan GR, Martin A, Straub J, Du Y, Simon VM, Poser S, Bush Z, Diaz J, Sahagun A, Gao J, Hernandez DG, Levine KS, Booth EO, Bateup HS, Rio DC, Hockemeyer D, Blauwendraat C, Soldner F. bioRxiv [Preprint]. 2024 Feb 13:2024.02.12.579917. doi: 10.1101/2024.02.12.579917. PubMed
Splice modulators target PMS1 to reduce somatic expansion of the Huntington's disease-associated CAG repeat. McLean ZL, Gao D, Correia K, Roy JCL, Shibata S, Farnum IN, Valdepenas-Mellor Z, Kovalenko M, Rapuru M, Morini E, Ruliera J, Gillis T, Lucente D, Kleinstiver BP, Lee JM, MacDonald ME, Wheeler VC, Mouro Pinto R, Gusella JF. Nat Commun. 2024 Apr 12;15(1):3182. doi: 10.1038/s41467-024-47485-0. PubMed

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