pYNL-N1_TUBB5
(Plasmid #65678)

• Purpose: Expresses beta-tubulin-YNL in mammalian cells
• Depositing Lab: Yasushi Okada
• Publication: Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.

Backbone

• Vector backbone: pYNL-N1
• Backbone manufacturer: Addgene #64303
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 6900
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-tubulin class V
• Alt name: TUBB5
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Promoter: CMV
• Tag / Fusion Protein:
  • YNL (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nano-lantern/pcDNA3 (gifted from Dr Takeharu Nagai)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry