Skip to main content
Addgene

FUW-ubiquitin-EphB2-SV40-GFP (B2G)
(Plasmid #65442)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    FUW
  • Backbone manufacturer
    Addgene Plasmid 14882
  • Total vector size (bp) 10365
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPHB2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2984
  • Mutation
    See depositor comments below.
  • Entrez Gene
    Ephb2 (a.k.a. Cek5, Drt, ETECK, Erk, Hek5, Nuk, Prkm5, Qek5, Sek3, Tyro5)
  • Promoter UBQ
  • Tag / Fusion Protein
    • SV40-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer hUBCpro-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein sequence for EphB2 matches NCBI AAB30284.1. Plasmid should function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-ubiquitin-EphB2-SV40-GFP (B2G) was a gift from Eduard Batlle (Addgene plasmid # 65442 ; http://n2t.net/addgene:65442 ; RRID:Addgene_65442)

  • For your References section:

    EphB-ephrin-B interactions suppress colorectal cancer progression by compartmentalizing tumor cells. Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, Lloreta J, Sancho E, Batlle E. Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30. 10.1038/ng.2007.11 PubMed 17906625
Related items:
Commonly requested with: