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Addgene

pBS-HxB.L
(Plasmid #65414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS (probably KS+)
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 10253
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TNC large splice variant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7292
  • Entrez Gene
    TNC (a.k.a. 150-225, DFNA56, GMEM, GP, HXB, JI, TN, TN-C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer catggtcatagctgtttcc
  • 3′ sequencing primer tcgaggtcgacggtatc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-HxB.L was a gift from Harold Erickson (Addgene plasmid # 65414 ; http://n2t.net/addgene:65414 ; RRID:Addgene_65414)

  • For your References section:

    Cell- and heparin-binding domains of the hexabrachion arm identified by tenascin expression proteins. Aukhil I, Joshi P, Yan Y, Erickson HP. J Biol Chem. 1993 Feb 5;268(4):2542-53. PubMed 7679097
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