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Addgene

pLXSN-EGFR
(Plasmid #65226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLXSN
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 9900
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EGFR
  • Alt name
    HER1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • GenBank ID
    NM_005228
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site HpaI (destroyed during cloning)
  • 5′ sequencing primer CTCTCCCCCTTGAACCTC
  • 3′ sequencing primer GACTTTCCACACCCTAACTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLXSN-EGFR was a gift from Axel Ullrich (Addgene plasmid # 65226 ; http://n2t.net/addgene:65226 ; RRID:Addgene_65226)

  • For your References section:

    Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation. Obermeier A, Tinhofer I, Grunicke HH, Ullrich A. EMBO J. 1996 Jan 2;15(1):73-82. PubMed 8598208
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