GFP-PELP1
(Plasmid #65208)

• Purpose: Full length mammalian expression vector for hPELP1
• Depositing Lab: Ratna Vadlamudi
• Publication: Nagpal et al Mol Cancer Res. 2008 May;6(5):851-61. doi: 10.1158/1541-7786.MCR-07-2030.

Backbone

• Vector backbone: pAcGFP1-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 8100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PELP1
• Alt name: proline, glutamate and leucine rich protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 3400
• Entrez Gene: PELP1 (a.k.a. MNAR, P160)
• Promoter: CMV
• Tag / Fusion Protein:
  • AcGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: sREACh-F (gaccccaacgagaagcgcg)
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry