pBAD18-Gm
(Plasmid
#65100)
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Purpose(Empty Backbone) bacterial expression vector with Gentamycin selection, based on pBAD18
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD18
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Backbone manufacturerBeckwith Lab
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Modifications to backboneChlor resistance gene replaced with Gentamycin resistance gene
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Vector typeBacterial Expression
- Promoter pBAD
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsgrow in 10ug/ul Gentamycin. Too much gentamycin is toxic for e. coli.
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBad-Fwd
- 3′ sequencing primer pBad-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD18-Gm was a gift from Juan Tomas (Addgene plasmid # 65100 ; http://n2t.net/addgene:65100 ; RRID:Addgene_65100)