pWPXL-resNP1
(Plasmid #65061)

• Purpose: Constitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1
• Depositing Lab: Ramon Trullas
• Publication: Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 10.1523/JNEUROSCI.2548-14.2015.

Backbone

• Vector backbone: pWPXL
• Backbone manufacturer: Didier Trono
• Backbone size w/o insert (bp): 10510
• Modifications to backbone: EGFP removed and replaced with insert.
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neuronal Pentraxin 1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1302
• Mutation: G447A, C450T, A451T, G452C, C454A, C456A, C459G
• GenBank ID: NM_153735.2
• Entrez Gene: Nptx1
• Promoter: EF1alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PmeI (destroyed during cloning)
• 3′ cloning site: PmeI (destroyed during cloning)
• 5′ sequencing primer: EF1a-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutations introduce a new EcoR1 restriction site on bp 459 of CDS to allow distinction between wildtype and resistant NP1. However, this prevents excision of the whole resNP1 insert from the pWPXL vector using EcoR1 restriction site