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Addgene

Mouse GFP-PRMT7 Wild Type
(Plasmid #65056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6779
  • Vector type
    Mammalian Expression ; Bacterial Propagation
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRMT7
  • Alt name
    Protein Arginine Methyltransferase 7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2079
  • GenBank ID
    NM_145404.1
  • Entrez Gene
    Prmt7 (a.k.a. 4933402B05Rik, BC006705)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mark Bedford, UT Texas MD Anderson

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results found an F4S amino acid change in the PRMT7 translation relative to genbank ID NM_145404.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse GFP-PRMT7 Wild Type was a gift from Steven Clarke (Addgene plasmid # 65056 ; http://n2t.net/addgene:65056 ; RRID:Addgene_65056)

  • For your References section:

    Mammalian protein arginine methyltransferase 7 (PRMT7) specifically targets RXR sites in lysine- and arginine-rich regions. Feng Y, Maity R, Whitelegge JP, Hadjikyriacou A, Li Z, Zurita-Lopez C, Al-Hadid Q, Clark AT, Bedford MT, Masson JY, Clarke SG. J Biol Chem. 2013 Dec 27;288(52):37010-25. doi: 10.1074/jbc.M113.525345. Epub 2013 Nov 18. 10.1074/jbc.M113.525345 PubMed 24247247
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