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Addgene

pTracer_Cr-TRP1
(Plasmid #64881)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64881 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8831

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transient receptor potential 1
  • Alt name
    Cr_TRP1
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
    3441
  • GenBank ID
    JX173491.1
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AfiII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTracer_Cr-TRP1 was a gift from Sebastián Brauchi (Addgene plasmid # 64881 ; http://n2t.net/addgene:64881 ; RRID:Addgene_64881)

  • For your References section:

    A transient receptor potential ion channel in Chlamydomonas shares key features with sensory transduction-associated TRP channels in mammals. Arias-Darraz L, Cabezas D, Colenso CK, Alegria-Arcos M, Bravo-Moraga F, Varas-Concha I, Almonacid DE, Madrid R, Brauchi S. Plant Cell. 2015 Jan;27(1):177-88. doi: 10.1105/tpc.114.131862. Epub 2015 Jan 16. 10.1105/tpc.114.131862 PubMed 25595824
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