pPM180
(Plasmid
#64822)
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PurposeGUS overexpression in planta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreenIIS BAR
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Modifications to backbone35S Promotor
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGUS
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Insert Size (bp)1800
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPM180 was a gift from Detlef Weigel (Addgene plasmid # 64822 ; http://n2t.net/addgene:64822 ; RRID:Addgene_64822) -
For your References section:
Temporal control of leaf complexity by miRNA-regulated licensing of protein complexes. Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, Baena-Gonzalez E, Wang JW, Weigel D. Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 10.1016/j.cub.2014.09.058. Epub 2014 Nov 6. 10.1016/j.cub.2014.09.058 PubMed 25448000