pTW040
(Plasmid #64769)

• Purpose: For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator
• Depositing Lab: Fred van Leeuwen
• Publication: Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.

Backbone

• Vector backbone: pRS303
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cre-EBD78
• Promoter: TDH3
• Tag / Fusion Protein:
  • fused to the human Estrogen Binding Domain (EBD), which makes the nuclear activity of Cre dependent on the hormone b-estradiol (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PvuII (destroyed during cloning)
• 3′ cloning site: PvuII (destroyed during cloning)
• 5′ sequencing primer: GPDpro-F
• 3′ sequencing primer: CYC1

Resource Information

• Supplemental Documents:
  • pTW040 annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry