pFGET19_Ulp1 Citations (21)
Originally described in: Tandem SUMO fusion vectors for improving soluble protein expression and purification.Guerrero F, Ciragan A, Iwai H Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. PubMed Journal
Articles Citing pFGET19_Ulp1
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| Strategies for over-expression and purification of recombinant full length STAT5B in Escherichia coli. de Araujo ED, Geletu M, Gunning PT. Protein Expr Purif. 2017 Jan;129:1-8. doi: 10.1016/j.pep.2016.08.017. Epub 2016 Aug 31. PubMed |
| Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers. Baranova N, Loose M. Methods Cell Biol. 2017;137:355-370. doi: 10.1016/bs.mcb.2016.03.036. Epub 2016 Jul 11. PubMed |
| High-throughput thermofluor-based assays for inhibitor screening of STAT SH2 domains. de Araujo ED, Manaswiyoungkul P, Israelian J, Park J, Yuen K, Farhangi S, Berger-Becvar A, Abu-Jazar L, Gunning PT. J Pharm Biomed Anal. 2017 Sep 5;143:159-167. doi: 10.1016/j.jpba.2017.04.052. Epub 2017 May 1. PubMed |
| A versatile expression vector for the growth and amplification of unmodified phage display polypeptides. Winton AJ, Baptiste JL, Allen MA. Protein Expr Purif. 2018 Apr 17;149:31-36. doi: 10.1016/j.pep.2018.04.008. PubMed |
| The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a. van Aelst K, Martinez-Santiago CJ, Cross SJ, Szczelkun MD. Genes (Basel). 2019 Feb 22;10(2). pii: genes10020169. doi: 10.3390/genes10020169. PubMed |
| A novel approach for production of an active N-terminally truncated Ulp1 (SUMO protease 1) catalytic domain from Escherichia coli inclusion bodies. Linova MY, Risor MW, Jorgensen SE, Mansour Z, Kaya J, Sigurdarson JJ, Enghild JJ, Karring H. Protein Expr Purif. 2020 Feb;166:105507. doi: 10.1016/j.pep.2019.105507. Epub 2019 Oct 4. PubMed |
| Fidelity of prespacer capture and processing is governed by the PAM-mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system. Yoganand KN, Muralidharan M, Nimkar S, Anand B. J Biol Chem. 2019 Dec 27;294(52):20039-20053. doi: 10.1074/jbc.RA119.009438. Epub 2019 Nov 20. PubMed |
| Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Patchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, Sappakhaw K, Leelahakorn N, Ruenkam T, Wongsatit T, Athipanyasilp N, Eiamthong B, Lakkanasirorat B, Phoodokmai T, Niljianskul N, Pakotiprapha D, Chanarat S, Homchan A, Tinikul R, Kamutira P, Phiwkaow K, Soithongcharoen S, Kantiwiriyawanitch C, Pongsupasa V, Trisrivirat D, Jaroensuk J, Wongnate T, Maenpuen S, Chaiyen P, Kamnerdnakta S, Swangsri J, Chuthapisith S, Sirivatanauksorn Y, Chaimayo C, Sutthent R, Kantakamalakul W, Joung J, Ladha A, Jin X, Gootenberg JS, Abudayyeh OO, Zhang F, Horthongkham N, Uttamapinant C. Nat Biomed Eng. 2020 Dec;4(12):1140-1149. doi: 10.1038/s41551-020-00603-x. Epub 2020 Aug 26. PubMed |
| Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. Liu J, Xue Z, Vann KR, Shi X, Kutateladze TG. STAR Protoc. 2020 Oct 28;1(3):100155. doi: 10.1016/j.xpro.2020.100155. eCollection 2020 Dec 18. PubMed |
Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonuclease.
Canal B, McClure AW, Curran JF, Wu M, Ulferts R, Weissmann F, Zeng J, Bertolin AP, Milligan JC, Basu S, Drury LS, Deegan TD, Fujisawa R, Roberts EL, Basier C, Labib K, Beale R, Howell M, Diffley JFX.
Biochem J. 2021 Jul 16;478(13):2445-2464. doi: 10.1042/BCJ20210198.
PubMed
Associated Plasmids |
| Incorporating thioamides into proteins by native chemical ligation. Fiore KE, Phan HAT, Robkis DM, Walters CR, Petersson EJ. Methods Enzymol. 2021;656:295-339. doi: 10.1016/bs.mie.2021.04.011. Epub 2021 May 7. PubMed |
| Identifying Distinct Structural Features of the SARS-CoV-2 Spike Protein Fusion Domain Essential for Membrane Interaction. Birtles D, Lee J. Biochemistry. 2021 Oct 12;60(40):2978-2986. doi: 10.1021/acs.biochem.1c00543. Epub 2021 Sep 27. PubMed |
| Imaging of anthrax intoxication in mice reveals shared and individual functions of surface receptors CMG-2 and TEM-8 in cellular toxin entry. Merritt C, Chun EM, Fattah RJ, Silva LM, Ma QQ, Moayeri M, Paliga D, Neumann S, Heumann R, Leppla SH, Bugge TH. J Biol Chem. 2022 Jan;298(1):101467. doi: 10.1016/j.jbc.2021.101467. Epub 2021 Dec 4. PubMed |
| Identification of fragments binding to SARS-CoV-2 nsp10 reveals ligand-binding sites in conserved interfaces between nsp10 and nsp14/nsp16. Kozielski F, Sele C, Talibov VO, Lou J, Dong D, Wang Q, Shi X, Nyblom M, Rogstam A, Krojer T, Fisher Z, Knecht W. RSC Chem Biol. 2021 Oct 6;3(1):44-55. doi: 10.1039/d1cb00135c. eCollection 2022 Jan 5. PubMed |
| Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states. Chen Z, Huang J, Li J. STAR Protoc. 2022 Aug 19;3(3):101642. doi: 10.1016/j.xpro.2022.101642. eCollection 2022 Sep 16. PubMed |
| Expression and kinetic characterization of PYCR3. Meeks KR, Tanner JJ. Arch Biochem Biophys. 2023 Jan 1;733:109468. doi: 10.1016/j.abb.2022.109468. Epub 2022 Nov 19. PubMed |
| Characterization of a unique polysaccharide monooxygenase from the plant pathogen Magnaporthe oryzae. Martinez-D'Alto A, Yan X, Detomasi TC, Sayler RI, Thomas WC, Talbot NJ, Marletta MA. Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2215426120. doi: 10.1073/pnas.2215426120. Epub 2023 Feb 15. PubMed |
| High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors. Hamaker NK, Lee KH. Biotechnol Bioeng. 2023 Sep;120(9):2419-2440. doi: 10.1002/bit.28393. Epub 2023 Apr 11. PubMed |
| Simple purification and characterization of soluble and homogenous ABC-F translation factors from Enterococcus faecium. Demay F, Hallier M, Georgeault S, Com E, Cattoir V, Goude R, Gillet R. Protein Expr Purif. 2023 Oct;210:106325. doi: 10.1016/j.pep.2023.106325. Epub 2023 Jun 22. PubMed |
| Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections. Mani S, Kaur A, Jakhar K, Kumari G, Sonar S, Kumar A, Das S, Kumar S, Kumar V, Kundu R, Pandey AK, Singh UP, Majumdar T. Int J Biol Macromol. 2023 Aug 1;245:125444. doi: 10.1016/j.ijbiomac.2023.125444. Epub 2023 Jun 27. PubMed |
Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3.
Singh AK, Krezel A.
Microb Cell Fact. 2023 Jul 11;22(1):125. doi: 10.1186/s12934-023-02134-x.
PubMed
Associated Plasmids |
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