pIRES Centrin1 mCherry
(Plasmid #64338)

• Purpose: Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene
• Depositing Lab: Matthieu Piel
• Publication: Piel Lab Plasmids (unpublished)

Backbone

• Vector backbone: pIREShyg3
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Centrin1
• Species: H. sapiens (human)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • IRES-Hygro (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry