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Addgene

human PNP non-tagged
(Plasmid #64199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-T7/CT-TOPO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Purine Nucleoside Phosphorylase
  • Alt name
    PNP
  • Species
    H. sapiens (human)
  • Entrez Gene
    PNP (a.k.a. NP, PRO1837, PUNP)
  • Tag / Fusion Protein
    • none

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human PNP non-tagged was a gift from Vern Schramm (Addgene plasmid # 64199 ; http://n2t.net/addgene:64199 ; RRID:Addgene_64199)

  • For your References section:

    Neighboring group participation in the transition state of human purine nucleoside phosphorylase. Murkin AS, Birck MR, Rinaldo-Matthis A, Shi W, Taylor EA, Almo SC, Schramm VL. Biochemistry. 2007 May 1;46(17):5038-49. Epub 2007 Apr 4. 10.1021/bi700147b PubMed 17407325
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