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Addgene

ROZA-XL
(Plasmid #64194)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA 3.1+
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5445
  • Total vector size (bp) 1846
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ROZA-XL
  • Species
    Synthetic
  • Insert Size (bp)
    1846
  • Mutation
    ROZA-XL
  • GenBank ID
    KM979358
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct includes a YFP varient "YPET" that I obtained from PS Daugherty, UC Davis, USA (see reference below) and a monomeric variant of CFP that was obtained from RY Tsien UC San Diego, USA (see reference below)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for CFP:
Zacharias DA, Violin JD, Newton AC, Tsien RY (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916.

Reference for YPET, yellow fluorescent protein:
A.W. Nguyen, P.S. Daugherty, Evolutionary optimization of fluorescent proteins for intracellular FRET, Nat. Biotechnol. 23 (2005) 355e360

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ROZA-XL was a gift from Annemarie Lellouch (Addgene plasmid # 64194 ; http://n2t.net/addgene:64194 ; RRID:Addgene_64194)

  • For your References section:

    ROZA-XL, an improved FRET based biosensor with an increased dynamic range for visualizing Zeta Associated Protein 70 kD (ZAP-70) tyrosine kinase activity in live T cells. Cadra S, Gucciardi A, Valignat MP, Theodoly O, Vacaflores A, Houtman JC, Lellouch AC. Biochem Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5. doi: 10.1016/j.bbrc.2015.02.117. 10.1016/j.bbrc.2015.02.117 PubMed 25735979
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