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Addgene

pKMD106e - intein-Cas9(S219)
(Plasmid #64190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJDS246
  • Backbone size w/o insert (bp) 3399
  • Total vector size (bp) 8850
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
  • Alt name
    intein-Cas9(S219)
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    5451
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS - 3x FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKMD106e - intein-Cas9(S219) was a gift from David Liu (Addgene plasmid # 64190 ; http://n2t.net/addgene:64190 ; RRID:Addgene_64190)

  • For your References section:

    Small molecule-triggered Cas9 protein with improved genome-editing specificity. Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR. Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6. 10.1038/nchembio.1793 PubMed 25848930
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