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Addgene

pHLUK
(Plasmid #64181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHLUM (version 2)
  • Modifications to backbone
    replaced MET17 with LYS2 (S288c background) removed a BamHI restriction site
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2, URA3, HIS3 ; LYS2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLUK was a gift from Markus Ralser (Addgene plasmid # 64181 ; http://n2t.net/addgene:64181 ; RRID:Addgene_64181)

  • For your References section:

    Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities. Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M. F1000Res. 2016 Sep 20;5:2351. eCollection 2016. 10.12688/f1000research.9606.1 PubMed 27830062
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