Luc-Sox2-3'UTR
(Plasmid
#63895)
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PurposeLuciferase reporter of mouse wildtype Sox2 3'UTR
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-Control
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5256
- Total vector size (bp) 6217
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Modifications to backboneatt L1 and L2 cassette were first cloned to XbaI site for Gateway cloning
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSRY (sex determining region Y)-box 2
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Alt namelcc; ysb; Sox-2
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SpeciesM. musculus (mouse)
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Insert Size (bp)961
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GenBank ID20674
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer GLprimer2
- 3′ sequencing primer RVprimer4 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Luc-Sox2-3'UTR was a gift from Lin He (Addgene plasmid # 63895 ; http://n2t.net/addgene:63895 ; RRID:Addgene_63895) -
For your References section:
miR-34 miRNAs provide a barrier for somatic cell reprogramming. Choi YJ, Lin CP, Ho JJ, He X, Okada N, Bu P, Zhong Y, Kim SY, Bennett MJ, Chen C, Ozturk A, Hicks GG, Hannon GJ, He L. Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366. 10.1038/ncb2366 PubMed 22020437