pRetroX GFP T2A Cre
(Plasmid #63704)

• Purpose: Inducible expression of GFP T2A Cre fusion in mammalian cells
• Depositing Lab: Floris Foijer
• Publication: Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 10.1073/pnas.1400892111. Epub 2014 Sep 2.

Backbone

• Vector backbone: pRetrox
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7305
• Total vector size (bp): 8424
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T2A Cre
• Alt name: Cre recombinase
• Insert Size (bp): 1124
• Promoter: tight TRE promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: MSCV-rev

Resource Information

• Supplemental Documents:
  • pRetroX GFP T2A Cre
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry