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PurposeAccepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSB1C3
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Backbone manufacturerBioBricks Foundation
- Backbone size w/o insert (bp) 2069
- Total vector size (bp) 3138
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRFP cloning selection cassette
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Alt nameLacI_monomeric red fluorescent protein_double terminator
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SpeciesSynthetic
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Insert Size (bp)1069
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GenBank IDAF506027.1
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byInsert parts came from iGEM/BioBricks foundation.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAP1 was a gift from Nicola Patron (Addgene plasmid # 63674 ; http://n2t.net/addgene:63674 ; RRID:Addgene_63674) -
For your References section:
Standards for plant synthetic biology: a common syntax for exchange of DNA parts. Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farre G, Rogers C, Smith A, Hibberd J, Webb AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loque D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ane JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JA, Ntoukakis V, Schafer P, Denby K, Edwards KJ, Osbourn A, Haseloff J. New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532. 10.1111/nph.13532 PubMed 26171760
Map uploaded by the depositor.
