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Addgene

pCMV2-hNCOA6:V5
(Plasmid #63585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/V5-HisB
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NCOA6
  • Alt name
    nuclear receptor coactivator 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6200
  • Entrez Gene
    NCOA6 (a.k.a. AIB3, ASC2, NRC, PRIP, RAP250, TRBP)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV2-hNCOA6:V5 was a gift from Kenneth Irvine (Addgene plasmid # 63585 ; http://n2t.net/addgene:63585 ; RRID:Addgene_63585)

  • For your References section:

    Yorkie promotes transcription by recruiting a histone methyltransferase complex. Oh H, Slattery M, Ma L, White KP, Mann RS, Irvine KD. Cell Rep. 2014 Jul 24;8(2):449-59. doi: 10.1016/j.celrep.2014.06.017. Epub 2014 Jul 10. 10.1016/j.celrep.2014.06.017 PubMed 25017066
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