pCMV NucleoBAS N354K-I372V
(Plasmid
#63574)
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PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63574 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepECFP-C1
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Backbone manufacturerClontech
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Modifications to backboneSynthetic DNA construct encoding BAS-1 was cloned into pECFP-C1, replacing ECFP, to generate NucleoBAS (SV40 NLS flanked by KpnI sites)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNucleoBAS N354K-I372V
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SpeciesSynthetic
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MutationN345K and I372V
- Promoter CMV
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Tag
/ Fusion Protein
- SV40 NLS (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40 poly A Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV NucleoBAS N354K-I372V was a gift from Stan van de Graaf (Addgene plasmid # 63574 ; http://n2t.net/addgene:63574 ; RRID:Addgene_63574) -
For your References section:
Monitoring bile acid transport in single living cells using a genetically encoded Forster resonance energy transfer sensor. van der Velden LM, Golynskiy MV, Bijsmans IT, van Mil SW, Klomp LW, Merkx M, van de Graaf SF. Hepatology. 2013 Feb;57(2):740-52. doi: 10.1002/hep.26012. Epub 2013 Jan 8. 10.1002/hep.26012 PubMed 22899095