pKG187
(Plasmid
#63077)
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PurposeRetroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIC242
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFAAP24, IMAGE clone 3659284
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Alt nameC19orf40
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SpeciesH. sapiens (human)
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Insert Size (bp)648
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Entrez GeneFAAP24 (a.k.a. C19orf40)
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Tags
/ Fusion Proteins
- EGFP (N terminal on backbone)
- TEV-S (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer oCB21
- 3′ sequencing primer oCB22 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKG187 was a gift from Iain Cheeseman (Addgene plasmid # 63077 ; http://n2t.net/addgene:63077 ; RRID:Addgene_63077)