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Addgene

pTpPuc3
(Plasmid #62864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Modifications to backbone
    Knockout of Bla gene with KanR from pACYC177, Addition of Thalassiosira pseudonana Nat cassette, yeast CEN6-ARSH4-HIS3, oriT from RP4
  • Vector type
    Synthetic Biology ; Episomal vector for Thalassiosira pseudonana
  • Selectable markers
    URA3, HIS3 ; Nat cassette for resistance in Thalassiosira pseudonana

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains yeast URA3 and HIS3 genes for selection in yeast. Contains Nat Cassette for nourseothricin resistance in Thalassiosira pseudonana.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTpPuc3 was a gift from Hamilton Smith (Addgene plasmid # 62864 ; http://n2t.net/addgene:62864 ; RRID:Addgene_62864)

  • For your References section:

    Designer diatom episomes delivered by bacterial conjugation. Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, Beeri K, Ellisman MH, Glass JI, Hutchison CA 3rd, Smith HO, Venter JC, Allen AE, Dupont CL, Weyman PD. Nat Commun. 2015 Apr 21;6:6925. doi: 10.1038/ncomms7925. 10.1038/ncomms7925 PubMed 25897682
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