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p0521s
(Plasmid #62862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCC1BAC
  • Modifications to backbone
    Addition of yeast Cen6-ArsH4-His3, P. tricornutum R1R2, origin of transfer from RP4, P. tricornutum ShBle cassette
  • Vector type
    Synthetic Biology ; Episomal vector for Phaeodactylum tricornutum
  • Selectable markers
    Zeocin, URA3, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Epi300 for inducible high copy expression
  • Copy number
    Low Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains yeast URA3 and HIS3 genes for selection in yeast. Contains ShBle cassette for selection in Phaeodactylum tricornutum.

Depositor states that the I-SceI restriction site in this plasmid (5’-AGTTACGCTAGGGATAACAGGTAATATAG) varies from the NEB listed recognition sequence and may reduce the efficiency of cleavage using this enzyme.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p0521s was a gift from Hamilton Smith (Addgene plasmid # 62862 ; http://n2t.net/addgene:62862 ; RRID:Addgene_62862)

  • For your References section:

    Designer diatom episomes delivered by bacterial conjugation. Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, Beeri K, Ellisman MH, Glass JI, Hutchison CA 3rd, Smith HO, Venter JC, Allen AE, Dupont CL, Weyman PD. Nat Commun. 2015 Apr 21;6:6925. doi: 10.1038/ncomms7925. 10.1038/ncomms7925 PubMed 25897682
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