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Purpose(Empty Backbone) To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV-SIBR-anti_I
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Backbone manufacturerChung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
- Backbone size (bp) 5235
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Modifications to backboneThe SIBR cassette is deleted and in the place of the eGFP a new polylinked was added.
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Vector typeMammalian Expression, AAV ; Eucaryotic
- Promoter hUbC
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer pCAG-F-primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-UbC was a gift from Jae Lee & Pantelis Tsoulfas (Addgene plasmid # 62806 ; http://n2t.net/addgene:62806 ; RRID:Addgene_62806) -
For your References section:
3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates. Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, Lemmon VP, Lee JK, Tsoulfas P. Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015. 10.1523/ENEURO.0001-15.2015 PubMed 26023683
