pAAV-UbC
(Plasmid #62806)

• Purpose: (Empty Backbone) To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.
• Depositing Labs: Jae Lee, Pantelis Tsoulfas
• Publication: Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAV-SIBR-anti_I
• Backbone manufacturer: Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
• Backbone size (bp): 5235
• Modifications to backbone: The SIBR cassette is deleted and in the place of the eGFP a new polylinked was added.
• Vector type: Mammalian Expression, AAV ; Eucaryotic
• Promoter: hUbC

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: pCAG-F-primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry