Actin-mRFP-PAGFP
(Plasmid #62382)

• Purpose: Expresses beta actin with both a PAGFP and mRFP tag.
• Depositing Labs: Guillaume Charras, Tim Mitchison
• Publication: Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53. doi: 10.1083/jcb.200801027.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: Clontech
• Modifications to backbone: Replaced EGFP by PAGFP Added mRFP to the N terminal of PAGFP between NheI and AgeI
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta actin
• Species: H. sapiens (human)
• Insert Size (bp): 1128
• GenBank ID: NM_001101.4
• Entrez Gene: ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mRFP (N terminal on insert)
  • PAGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: EGFP C rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry