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PurposePlasmid for tet-inducible expression of dCas9-EGFP in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript II SK (+)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-EGFP
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SpeciesSynthetic
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Insert Size (bp)4995
- Promoter TRE
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- SV40 NLS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13/pUC Forward
- 3′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSKΔB-TRE-dCas9-EGFP was a gift from Hiroshi Ochiai (Addgene plasmid # 62347 ; http://n2t.net/addgene:62347 ; RRID:Addgene_62347) -
For your References section:
Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. 10.1093/nar/gkv624 PubMed 26092696
Map uploaded by the depositor.
