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Addgene

pLenti SpBsmBI sgRNA Hygro
(Plasmid #62205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Cloning Grade DNA 62205-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105
Add to Cart

Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    Addgene plasmid number: 36297
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter U6
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Information for Cloning Grade DNA (Catalog # 62205-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti SpBsmBI sgRNA Hygro was a gift from Rene Maehr (Addgene plasmid # 62205 ; http://n2t.net/addgene:62205 ; RRID:Addgene_62205)

  • For your References section:

    Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Pham H, Kearns NA, Maehr R. Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3. 10.1007/978-1-4939-3067-8_3 PubMed 26463376
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