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NET39 mRFP-C1 (1219)
(Plasmid #62042)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mRFP-C1
  • Backbone manufacturer
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NET39
  • Species
    H. sapiens (human)
  • GenBank ID
    AAH06362.2
  • Entrez Gene
    PPAPDC3 (a.k.a. C9orf67, NET39)
  • Promoter CMV
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer RFP-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NET39 mRFP-C1 (1219) was a gift from Eric Schirmer (Addgene plasmid # 62042 ; http://n2t.net/addgene:62042 ; RRID:Addgene_62042)

  • For your References section:

    System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Zuleger N, Kelly DA, Richardson AC, Kerr AR, Goldberg MW, Goryachev AB, Schirmer EC. J Cell Biol. 2011 Apr 4;193(1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. 10.1083/jcb.201009068 PubMed 21444689
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