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Addgene

Sec61 beta pAcGFP-C1 (802)
(Plasmid #62008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec61 beta
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEC61B
  • Promoter CMV
  • Tag / Fusion Protein
    • AcGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer AcGFP1-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sec61 beta pAcGFP-C1 (802) was a gift from Eric Schirmer (Addgene plasmid # 62008 ; http://n2t.net/addgene:62008 ; RRID:Addgene_62008)

  • For your References section:

    System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Zuleger N, Kelly DA, Richardson AC, Kerr AR, Goldberg MW, Goryachev AB, Schirmer EC. J Cell Biol. 2011 Apr 4;193(1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. 10.1083/jcb.201009068 PubMed 21444689
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