CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
(Plasmid #61762)

• Purpose: Dual Color betaGlobin reporter to study splicing kinetics in vivo
• Depositing Lab: Daniel Larson
• Publication: Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939.

Backbone

• Vector backbone: Tet-ON
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3470
• Total vector size (bp): 8669
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: b-Globin
• Species: H. sapiens (human)
• Insert Size (bp): 2860
• Promoter: Dox inducible Tet-ON promoter
• Tags / Fusion Proteins:
  • 24 X PP7 hairpin cassette in intron 2 of b-globin gene
  • CFP-SKL (C terminal on insert)
  • 24 X MS2 hairpin cassette in 3'UTR of b-Globin reporter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • CFP_betaGlobin_24XPP7_24XMS2_reporter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry