Skip to main content
Addgene

pFPL-Yh2
(Plasmid #61650)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCAMBIA1300
  • Backbone manufacturer
    Cambia, Canberra, Australia
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 11500
  • Modifications to backbone
    Additions to T-DNA region: insertion of selectable marker (hygromycin resistance), gateway cassette, GFP gene, fungal terminator sequences.
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    enhanced yellow fluorescent protein
  • Alt name
    eYFP
  • Species
    Auquorea victoria
  • Insert Size (bp)
    720
  • GenBank ID
    JX472996.1

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFPL-Yh2 was a gift from Mark Farman (Addgene plasmid # 61650 ; http://n2t.net/addgene:61650 ; RRID:Addgene_61650)

  • For your References section:

    pFPL vectors for high-throughput protein localization in fungi: detecting cytoplasmic accumulation of putative effector proteins. Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin MM, Farman M. Mol Plant Microbe Interact. 2014 Nov 12. 10.1094/MPMI-05-14-0144-TA PubMed 25390188
Related items:
Commonly requested with: