pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
(Plasmid #61591)

• Purpose: A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
• Depositing Lab: Feng Zhang
• Publication: Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature14299.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hSaCas9
• Alt name: hSauCas9
• Species: Staphylococcus aureus
• Insert Size (bp): 3345
• GenBank ID: CCK74173.1
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • 3xHA (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Chimeric guide for SaCas9
• Species: Staphylococcus aureus
• Insert Size (bp): 101

Resource Information

• Supplemental Documents:
  • pX601-CMV-SaCas9(reopt)-3xHA-U6-BsaI(sgRNA).gbk
  • SaCas9 User Manual
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 129 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry