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Addgene

GST-PRMT7 LDIG69AAAA
(Plasmid #61560)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61560 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX(SN)
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRMT7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Mutation
    LDIG 69-72 AAAA
  • Entrez Gene
    PRMT7 (a.k.a. SBIDDS)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PRMT7 LDIG69AAAA was a gift from Steven Clarke (Addgene plasmid # 61560 ; http://n2t.net/addgene:61560 ; RRID:Addgene_61560)

  • For your References section:

    Substrate Specificity of Human Protein Arginine Methyltransferase 7 (PRMT7): THE IMPORTANCE OF ACIDIC RESIDUES IN THE DOUBLE E LOOP. Feng Y, Hadjikyriacou A, Clarke SG. J Biol Chem. 2014 Nov 21;289(47):32604-16. doi: 10.1074/jbc.M114.609271. Epub 2014 Oct 7. 10.1074/jbc.M114.609271 PubMed 25294873
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