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Addgene

RAB14 shRNA
(Plasmid #61496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.3G
  • Backbone manufacturer
    Christophe Benoist & Diane Mathis (Addgene plasmid # 14748)
  • Backbone size w/o insert (bp) 8174
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RAB14
  • gRNA/shRNA sequence
    TAACAGCCCTAAATCGCTCCT
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    RAB14 (a.k.a. FBP, RAB-14)
  • Promoter hU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer LKO.1 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RAB14 shRNA was a gift from Curt Civin (Addgene plasmid # 61496 ; http://n2t.net/addgene:61496 ; RRID:Addgene_61496)

  • For your References section:

    MIR144 and MIR451 regulate human erythropoiesis via RAB14. Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI. Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164. 10.1111/bjh.13164 PubMed 25312678
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