pMDC32B-OsMIR390-B/c
(Plasmid #61467)

• Purpose: Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
• Depositing Lab: James Carrington
• Publication: Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.

Backbone

• Vector backbone: pMDC32
• Backbone size w/o insert (bp): 10145
• Total vector size (bp): 11675
• Modifications to backbone: A BsaI site of the backbone was mutated to block the site.
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsMIR390-B/c
• Species: Oryza sativa
• Insert Size (bp): 1530
• Mutation: O. sativa MIR390 precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.
• Promoter: 2x35S

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: attB1
• 3′ sequencing primer: attB2

Resource Information

• Supplemental Documents:
  • Annotated sequence pMDC32B-OsMIR390-B/c
  • GenBank file pMDC32B-OsMIR390-B/c
  • Cloning protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry