sgRNA(MS2) cloning backbone Citations (54)
Originally described in: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136. PubMed Journal
Articles Citing sgRNA(MS2) cloning backbone
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| Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed |
Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9.
Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS.
PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016.
PubMed
Associated Plasmids |
| CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci. Fu Y, Rocha PP, Luo VM, Raviram R, Deng Y, Mazzoni EO, Skok JA. Nat Commun. 2016 May 25;7:11707. doi: 10.1038/ncomms11707. PubMed |
| Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems. Bialek JK, Dunay GA, Voges M, Schafer C, Spohn M, Stucka R, Hauber J, Lange UC. PLoS One. 2016 Jun 24;11(6):e0158294. doi: 10.1371/journal.pone.0158294. eCollection 2016. PubMed |
| Inhibition of histone deacetylase reduces transcription of NADPH oxidases and ROS production and ameliorates pulmonary arterial hypertension. Chen F, Li X, Aquadro E, Haigh S, Zhou J, Stepp DW, Weintraub NL, Barman SA, Fulton DJ. Free Radic Biol Med. 2016 Oct;99:167-178. doi: 10.1016/j.freeradbiomed.2016.08.003. Epub 2016 Aug 3. PubMed |
| Epigenetic Regulation of Interleukin 6 by Histone Acetylation in Macrophages and Its Role in Paraquat-Induced Pulmonary Fibrosis. Hu L, Yu Y, Huang H, Fan H, Hu L, Yin C, Li K, Fulton DJ, Chen F. Front Immunol. 2017 Jan 30;7:696. doi: 10.3389/fimmu.2016.00696. eCollection 2016. PubMed |
| Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Ferry QR, Lyutova R, Fulga TA. Nat Commun. 2017 Mar 3;8:14633. doi: 10.1038/ncomms14633. PubMed |
| The long noncoding RNA Wisper controls cardiac fibrosis and remodeling. Micheletti R, Plaisance I, Abraham BJ, Sarre A, Ting CC, Alexanian M, Maric D, Maison D, Nemir M, Young RA, Schroen B, Gonzalez A, Ounzain S, Pedrazzini T. Sci Transl Med. 2017 Jun 21;9(395). pii: 9/395/eaai9118. doi: 10.1126/scitranslmed.aai9118. PubMed |
| CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed |
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
PubMed
Associated Plasmids |
| Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA. Lundh M, Plucinska K, Isidor MS, Petersen PSS, Emanuelli B. Mol Metab. 2017 Oct;6(10):1313-1320. doi: 10.1016/j.molmet.2017.07.001. Epub 2017 Jul 8. PubMed |
| Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Kleinjan DA, Wardrope C, Nga Sou S, Rosser SJ. Nat Commun. 2017 Oct 30;8(1):1191. doi: 10.1038/s41467-017-01222-y. PubMed |
| Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM. Gene. 2018 Feb 5;642:188-198. doi: 10.1016/j.gene.2017.11.033. Epub 2017 Nov 14. PubMed |
| A transcribed enhancer dictates mesendoderm specification in pluripotency. Alexanian M, Maric D, Jenkinson SP, Mina M, Friedman CE, Ting CC, Micheletti R, Plaisance I, Nemir M, Maison D, Kernen J, Pezzuto I, Villeneuve D, Burdet F, Ibberson M, Leib SL, Palpant NJ, Hernandez N, Ounzain S, Pedrazzini T. Nat Commun. 2017 Nov 27;8(1):1806. doi: 10.1038/s41467-017-01804-w. PubMed |
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.
Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC.
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.
PubMed
Associated Plasmids |
Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing.
Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y.
Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222.
PubMed
Associated Plasmids |
| Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Chen CD, Zeldich E, Li Y, Yuste A, Abraham CR. J Mol Neurosci. 2018 Feb;64(2):175-184. doi: 10.1007/s12031-017-1011-0. Epub 2018 Jan 19. PubMed |
| lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures. Zhao Z, Senturk N, Song C, Grummt I. Genes Dev. 2018 Jun 1;32(11-12):836-848. doi: 10.1101/gad.311688.118. Epub 2018 Jun 15. PubMed |
| Biased genome editing using the local accumulation of DSB repair molecules system. Nakade S, Mochida K, Kunii A, Nakamae K, Aida T, Tanaka K, Sakamoto N, Sakuma T, Yamamoto T. Nat Commun. 2018 Aug 16;9(1):3270. doi: 10.1038/s41467-018-05773-6. PubMed |
| CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles. Yamazaki T, Fujikawa C, Kubota A, Takahashi A, Hirose T. Biochem Biophys Res Commun. 2018 Sep 26;504(1):218-224. doi: 10.1016/j.bbrc.2018.08.158. Epub 2018 Sep 1. PubMed |
| Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements. Thormann V, Glaser LV, Rothkegel MC, Borschiwer M, Bothe M, Fuchs A, Meijsing SH. Life Sci Alliance. 2019 Mar 13;2(2). pii: 2/2/e201800283. doi: 10.26508/lsa.201800283. Print 2019 Apr. PubMed |
CRISPRai for simultaneous gene activation and inhibition to promote stem cell chondrogenesis and calvarial bone regeneration.
Truong VA, Hsu MN, Kieu Nguyen NT, Lin MW, Shen CC, Lin CY, Hu YC.
Nucleic Acids Res. 2019 Jul 26;47(13):e74. doi: 10.1093/nar/gkz267.
PubMed
Associated Plasmids |
| Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes. Ferdosi SR, Ewaisha R, Moghadam F, Krishna S, Park JG, Ebrahimkhani MR, Kiani S, Anderson KS. Nat Commun. 2019 Apr 23;10(1):1842. doi: 10.1038/s41467-019-09693-x. PubMed |
| A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9. Maji B, Gangopadhyay SA, Lee M, Shi M, Wu P, Heler R, Mok B, Lim D, Siriwardena SU, Paul B, Dancik V, Vetere A, Mesleh MF, Marraffini LA, Liu DR, Clemons PA, Wagner BK, Choudhary A. Cell. 2019 May 2;177(4):1067-1079.e19. doi: 10.1016/j.cell.2019.04.009. PubMed |
| Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors. Tran NT, Bashir S, Li X, Rossius J, Chu VT, Rajewsky K, Kuhn R. Front Genet. 2019 Apr 30;10:365. doi: 10.3389/fgene.2019.00365. eCollection 2019. PubMed |
| The lncRNA Locus Handsdown Regulates Cardiac Gene Programs and Is Essential for Early Mouse Development. Ritter N, Ali T, Kopitchinski N, Schuster P, Beisaw A, Hendrix DA, Schulz MH, Muller-McNicoll M, Dimmeler S, Grote P. Dev Cell. 2019 Sep 9;50(5):644-657.e8. doi: 10.1016/j.devcel.2019.07.013. Epub 2019 Aug 15. PubMed |
| CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration. Hsu MN, Liao HT, Truong VA, Huang KL, Yu FJ, Chen HH, Nguyen TKN, Makarevich P, Parfyonova Y, Hu YC. Theranostics. 2019 Aug 14;9(21):6099-6111. doi: 10.7150/thno.36790. eCollection 2019. PubMed |
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs.
Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E.
Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5.
PubMed
Associated Plasmids
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| Automated 3D light-sheet screening with high spatiotemporal resolution reveals mitotic phenotypes. Eismann B, Krieger TG, Beneke J, Bulkescher R, Adam L, Erfle H, Herrmann C, Eils R, Conrad C. J Cell Sci. 2020 Jun 1;133(11). pii: jcs.245043. doi: 10.1242/jcs.245043. PubMed |
| Decipher the complexity of cis-regulatory regions by a modified Cas9. Kirchner S, Reuter S, Westphal A, Mrowka R. PLoS One. 2020 Jul 2;15(7):e0235530. doi: 10.1371/journal.pone.0235530. eCollection 2020. PubMed |
| Synthetic immunomodulation with a CRISPR super-repressor in vivo. Moghadam F, LeGraw R, Velazquez JJ, Yeo NC, Xu C, Park J, Chavez A, Ebrahimkhani MR, Kiani S. Nat Cell Biol. 2020 Sep;22(9):1143-1154. doi: 10.1038/s41556-020-0563-3. Epub 2020 Sep 3. PubMed |
| Harnessing tRNA for Processing Ability and Promoter Activity. Knapp DJHF, Fulga TA. Methods Mol Biol. 2021;2162:89-114. doi: 10.1007/978-1-0716-0687-2_7. PubMed |
| Identification of human long noncoding RNAs associated with nonalcoholic fatty liver disease and metabolic homeostasis. Ruan X, Li P, Ma Y, Jiang CF, Chen Y, Shi Y, Gupta N, Seifuddin F, Pirooznia M, Ohnishi Y, Yoneda N, Nishiwaki M, Dumbovic G, Rinn JL, Higuchi Y, Kawai K, Suemizu H, Cao H. J Clin Invest. 2021 Jan 4;131(1). pii: 136336. doi: 10.1172/JCI136336. PubMed |
| Identification and characterization of a new isoform of small GTPase RhoE. Dai Y, Luo W, Yue X, Ma W, Wang J, Chang J. Commun Biol. 2020 Oct 15;3(1):572. doi: 10.1038/s42003-020-01295-4. PubMed |
| Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids. Velazquez JJ, LeGraw R, Moghadam F, Tan Y, Kilbourne J, Maggiore JC, Hislop J, Liu S, Cats D, Chuva de Sousa Lopes SM, Plaisier C, Cahan P, Kiani S, Ebrahimkhani MR. Cell Syst. 2021 Jan 20;12(1):41-55.e11. doi: 10.1016/j.cels.2020.11.002. Epub 2020 Dec 7. PubMed |
| Transcriptional behavior of the HIV-1 promoter in context of the BACH2 prominent proviral integration gene. Hamann MV, Ehmele P, Verdikt R, Bialek-Waldmann JK, Virdi S, Gunther T, Van Lint C, Grundhoff A, Hauber J, Lange UC. Virus Res. 2021 Feb;293:198260. doi: 10.1016/j.virusres.2020.198260. Epub 2020 Dec 11. PubMed |
| Activation of the miR-371/372/373 miRNA Cluster Enhances Oncogenicity and Drug Resistance in Oral Carcinoma Cells. Lin SC, Wu HL, Yeh LY, Yang CC, Kao SY, Chang KW. Int J Mol Sci. 2020 Dec 11;21(24). pii: ijms21249442. doi: 10.3390/ijms21249442. PubMed |
| CD46 splice variant enhances translation of specific mRNAs linked to an aggressive tumor cell phenotype in bladder cancer. Zeng J, Xu H, Huang C, Sun Y, Xiao H, Yu G, Zhou H, Zhang Y, Yao W, Xiao W, Hu J, Wu L, Xing J, Wang T, Chen Z, Ye Z, Chen K. Mol Ther Nucleic Acids. 2021 Feb 24;24:140-153. doi: 10.1016/j.omtn.2021.02.019. eCollection 2021 Jun 4. PubMed |
| Transcriptional Activation of Heterochromatin by Recruitment of dCas9 Activators. Frank L, Weinmann R, Erdel F, Trojanowski J, Rippe K. Methods Mol Biol. 2021;2351:307-320. doi: 10.1007/978-1-0716-1597-3_17. PubMed |
| Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors. Cebola I. Methods Mol Biol. 2021;2351:321-334. doi: 10.1007/978-1-0716-1597-3_18. PubMed |
| Inhibition of the NOTCH1 Pathway in the Stressed Heart Limits Fibrosis and Promotes Recruitment of Non-Myocyte Cells into the Cardiomyocyte Fate. Nemir M, Kay M, Maison D, Berthonneche C, Sarre A, Plaisance I, Pedrazzini T. J Cardiovasc Dev Dis. 2022 Apr 7;9(4). pii: jcdd9040111. doi: 10.3390/jcdd9040111. PubMed |
| Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA Gm15551. Engelhard CA, Huang C, Khani S, Kasparek P, Prochazka J, Rozman J, Reguera DP, Sedlacek R, Kornfeld JW. Noncoding RNA. 2022 May 6;8(3):32. doi: 10.3390/ncrna8030032. PubMed |
| YAP1 induces invadopodia formation by transcriptionally activating TIAM1 through enhancer in breast cancer. Shen J, Huang Q, Jia W, Feng S, Liu L, Li X, Tao D, Xie D. Oncogene. 2022 Jul;41(31):3830-3845. doi: 10.1038/s41388-022-02344-4. Epub 2022 Jun 30. PubMed |
| Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB. Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. PubMed |
| A Ctnnb1 enhancer regulates neocortical neurogenesis by controlling the abundance of intermediate progenitors. Wang J, Wang A, Tian K, Hua X, Zhang B, Zheng Y, Kong X, Li W, Xu L, Wang J, Li Z, Liu Y, Zhou Y. Cell Discov. 2022 Aug 2;8(1):74. doi: 10.1038/s41421-022-00421-2. PubMed |
| Arc Regulates Transcription of Genes for Plasticity, Excitability and Alzheimer's Disease. Leung HW, Foo G, VanDongen A. Biomedicines. 2022 Aug 11;10(8):1946. doi: 10.3390/biomedicines10081946. PubMed |
| Long non-coding RNA PCAT19 safeguards DNA in quiescent endothelial cells by preventing uncontrolled phosphorylation of RPA2. Oo JA, Palfi K, Warwick T, Wittig I, Prieto-Garcia C, Matkovic V, Tomaskovic I, Boos F, Izquierdo Ponce J, Teichmann T, Petriukov K, Haydar S, Maegdefessel L, Wu Z, Pham MD, Krishnan J, Baker AH, Gunther S, Ulrich HD, Dikic I, Leisegang MS, Brandes RP. Cell Rep. 2022 Nov 15;41(7):111670. doi: 10.1016/j.celrep.2022.111670. PubMed |
| Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation. Jin Q, Liu X, Zhuang Z, Huang J, Gou S, Shi H, Zhao Y, Ouyang Z, Liu Z, Li L, Mao J, Ge W, Chen F, Yu M, Guan Y, Ye Y, Tang C, Huang R, Wang K, Lai L. Genome Biol. 2023 Jan 17;24(1):8. doi: 10.1186/s13059-023-02851-x. PubMed |
| Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma. Sgro A, Cursons J, Waryah C, Woodward EA, Foroutan M, Lyu R, Yeoh GCT, Leedman PJ, Blancafort P. Clin Epigenetics. 2023 Apr 29;15(1):73. doi: 10.1186/s13148-023-01482-0. PubMed |
| Fendrr synergizes with Wnt signalling to regulate fibrosis related genes during lung development via its RNA:dsDNA triplex element. Ali T, Rogala S, Krause NM, Bains JK, Melissari MT, Wahrisch S, Schwalbe H, Herrmann BG, Grote P. Nucleic Acids Res. 2023 Jul 7;51(12):6227-6237. doi: 10.1093/nar/gkad395. PubMed |
| Functional mapping of microRNA promoters with dCas9 fused to transcriptional regulators. Kumar P, Courtes M, Lemmers C, Le Digarcher A, Coku I, Monteil A, Hong C, Varrault A, Liu R, Wang L, Bouschet T. Front Genet. 2023 May 5;14:1147222. doi: 10.3389/fgene.2023.1147222. eCollection 2023. PubMed |
| Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome. Engelhard CA, Khani S, Derdak S, Bilban M, Kornfeld JW. iScience. 2023 Jun 23;26(8):107190. doi: 10.1016/j.isci.2023.107190. eCollection 2023 Aug 18. PubMed |
| CRISPR-Hybrid: A CRISPR-Mediated Intracellular Directed Evolution Platform for RNA Aptamers. Su-Tobon Q, Fan J, Feeney K, Ren H, Autissier P, Wang P, Huang Y, Niu J. bioRxiv [Preprint]. 2023 Aug 30:2023.08.29.555185. doi: 10.1101/2023.08.29.555185. PubMed |
| The upregulation of VGF enhances the progression of oral squamous carcinoma. Chou CH, Yen CH, Liu CJ, Tu HF, Lin SC, Chang KW. Cancer Cell Int. 2024 Mar 25;24(1):115. doi: 10.1186/s12935-024-03301-9. PubMed |
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